Coding

Part:BBa_K3817001

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)


CkTcS new_enzyme

In the degradation of caffeine, two key processes include C8 oxidation and N9-methylation. CkTcS possesses novel N9-methyltransferase activity using 1,3,7-trimethyluric acid as a substrate. The intermediate product is converted to theacrine, catalyzed by CkTcS. We used Pross to predict and modify the heat resistance of the original enzyme so that its optimum temperature could be increased to 60 ℃. This part is the coding sequence of CkTcS after modifying.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 422
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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