In the degradation of caffeine, two key processes include C8 oxidation and N9-methylation. CkTcS possesses novel N9-methyltransferase activity using 1,3,7-trimethyluric acid as a substrate. The intermediate product is converted to theacrine, catalyzed by CkTcS. We used Pross to predict and modify the heat resistance of the original enzyme so that its optimum temperature could be increased to 60 ℃. This part is the coding sequence of CkTcS after modifying.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 6
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 422
- 1000COMPATIBLE WITH RFC