In the degradation of caffeine, two key processes include C8 oxidation and N9-methylation. CkTcS possesses novel N9-methyltransferase activity using 1,3,7-trimethyluric acid as a substrate. The intermediate product is converted to theacrine, catalyzed by CkTcS. This part is the coding sequence of CkTcS.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 6
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 425
- 1000COMPATIBLE WITH RFC