CecropineA-Mxe GryA intein-PT-linker-His tag
Description of this part
This part is for the purification of the antimicrobial peptide, CecropinA. This is derived from Hyalophora cecropia. This can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.
This part is composed of His tag, Mxe GyrA intein, PT linker, and targeted protein. This is for the peptide intein tag purification. Producing peptide with His tag, it is recovered by Ni chromatography. After that, adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we get the targeted protein.
Sequence and Features Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 67
Illegal AgeI site found at 346
- 1000COMPATIBLE WITH RFC
- Cells were grown in 1000ml LB media at 37oC shaking at 180 rpm.
- when the OD exceeded 0.35, 1 M IPTG 200μL was added to induce the peptide expression.
- Incubate at 30℃ at 180rpm for 6 hours after adding IPTG.
1.When this fused protein were produced, it was recovered by Ni chromatography.
2.Adding DTT to it, the targeted protein was cut out by the cleavage of intein.
3.SDSPAGE was performed in order to confirm the presence of it.
Fig1 shows the result of SDS-PAGE. The lane 1 and 6 are the result of CecropinA.
CecropinA is 4051Da, so these date shows that we could not confirm CecA production.
1.Steiner, H., Andreu, D., and Merrifield, R.B. (1988). Binding and action of cecropin and cecropin analogues: antibacterial peptides from insects. Biochim. Biophys. Acta 939, 260–266.