Part:BBa_K3813029
Mucin Protein-Coding Sequence
This part consists of the protein-coding sequence for Mucin-1(MUC-1), a biomarker that is used in the detection of breast cancer. Mucin-1 is usually found in the epithelial cells of the stomach and lungs, and over expression of Mucin-1 is often an indicator of breast cancer. The protein-coding sequence from this part was found via NCBI but we adapted the sequence to meet iGEM registry standards by inducing silent mutations that would not affect the sequence of amino acids. We used this part to construct a plasmid that would produce Mucin-1 in an E.Coli system to model the conditions of the body of patients with breast cancer.
To see the application of this part, please see the following composite parts.
BBa_K3813000, BBa_K3813003, BBa_K3813005, BBa_K3813006, BBa_K3813007, BBa_K3813008, BBa_K3813009, BBa_K3813010
We were successfully able to express the Mucin-1 biomarker in 7 of the 9 constructs. For the second phase of our two-year project, we plan on testing the binding affinity of Mucin-1 to our aptamers to better simulate situations in the human body and will hopefully use results from there to improve this part.
ASIJ_Tokyo has nominated the following for the Best Basic Part due to our contributions for adopting this part to iGEM registry strands. Since Mucin-1 is a versatile protein and biomarker, by making it universal through iGEM assembly standards, future teams will be able to apply this part in their own studies.
References
[1]Homo sapiens chromosome 1, GRCh38.p13 Primary Assembly - Nucleotide - NCBI. (n.d.). Retrieved August 2, 2021, from https://www.ncbi.nlm.nih.gov/nuccore/NC_000001.11?report=fasta&from=155185824&to=155192915&strand=true
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 6372
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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