Coding

Part:BBa_K3812016

Designed by: Guanghui Dong   Group: iGEM21_CHINA-FAFU   (2021-10-21)


DHAR overexpression system

In order to better optimize BBa_K3812008 so that DHAR can be better expressed, with the help of AilurusTM, we connect BBa_K3812001 to 2500 expression vectors and introduce them into DH5α for expression. Finally, we screened out the best expression system for DHAR-BBa_K3812016. BBa_K3812016 contains promoter-BBa_J56015, RBS-BBa_K3812011, 6xHis-tag-BBa_K3812012, DHAR coding sequence-BBa_K3812001, terminator-BBa_K3812013, plasmid backbone-BBa_K3812014 Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3104
    Illegal suffix found in sequence at 1056
    Illegal PstI site found at 209
    Illegal PstI site found at 772
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3104
    Illegal SpeI site found at 1057
    Illegal PstI site found at 209
    Illegal PstI site found at 772
    Illegal PstI site found at 1071
    Illegal NotI site found at 1064
    Illegal NotI site found at 3110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3104
    Illegal BamHI site found at 538
    Illegal XhoI site found at 2088
    Illegal XhoI site found at 2980
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3104
    Illegal suffix found in sequence at 1057
    Illegal PstI site found at 209
    Illegal PstI site found at 772
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3104
    Illegal XbaI site found at 3119
    Illegal SpeI site found at 1057
    Illegal PstI site found at 209
    Illegal PstI site found at 772
    Illegal PstI site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]


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