Composite

Part:BBa_K3812010

Designed by: Guanghui Dong   Group: iGEM21_CHINA-FAFU   (2021-10-01)


Code GDP-L-Galactose Phosphorylase（GGP）

Paox1 Promoter can regulate the initiation of VTC2 transcription, and CYC1 can terminate the transcription of VTC2. This conforming part can express VTC2 in the chassis organism-Pichia pastoris.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2859
    Illegal SpeI site found at 1673
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2859
    Illegal SpeI site found at 1673
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2859
    Illegal BglII site found at 1
    Illegal BglII site found at 1225
    Illegal BamHI site found at 1297
    Illegal BamHI site found at 4127
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2859
    Illegal SpeI site found at 1673
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2859
    Illegal SpeI site found at 1673
    Illegal NgoMIV site found at 2888
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None