Coding

Part:BBa_K3812001

Designed by: Guanghui Dong   Group: iGEM21_CHINA-FAFU   (2021-09-30)


Code dehydroascorbate reductase

BBa_K3812001 can encode DHAR.DHAR exists in chloroplast, mitochondria and cytoplasm. It is an important enzyme in the AsA-GSH cycle, which catalyzes the reduction of dehydroascorbic acid (DHA) by GSH and reduces DHA to AsA. It plays an important role in maintaining the reducing ability of ascorbic acid in plants. Transgenic plants overexpressing DHAR have reduced oxidative metabolism-related enzyme activities, increased chlorophyll content, and increased photosynthetic efficiency in an ozone environment, while transgenic plants that inhibit DHAR expression have the opposite result.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 40
    Illegal PstI site found at 603
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 40
    Illegal PstI site found at 603
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 40
    Illegal PstI site found at 603
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 40
    Illegal PstI site found at 603
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None