Coding

Part:BBa_K3807036

Designed by: Sarah Vorsselmans   Group: iGEM21_KU_Leuven   (2021-10-01)


gRNA scaffold with riboswitch protospacer 3

gRNA scaffold targeting the theophylline riboswitch (BBa_K598005). The protospacer targets the 5' end of the TetA gene directly downstream of the riboswitch. Mutations will be introduced in the upstream sequence, which covers the riboswitch sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None