Composite

Part:BBa_K3807034

Designed by: Viktoriia Belousova   Group: iGEM21_KU_Leuven   (2021-10-01)


pGGK-p35S-NLS-N7-enCas9-PolI3M-P2A-mCherry-sacB

pGG plasmid containing Evolvr enCas9-PolI3M expression cassette (BBa_K380707031) followed by a SacB promoter and SacB gene flanked by AarI restriction sites so that it can be easily substituted by a custom gRNA expression cassette

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6075
    Illegal EcoRI site found at 7974
    Illegal EcoRI site found at 19417
    Illegal XbaI site found at 10380
    Illegal PstI site found at 6102
    Illegal PstI site found at 7554
    Illegal PstI site found at 10344
    Illegal PstI site found at 10356
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6075
    Illegal EcoRI site found at 7974
    Illegal EcoRI site found at 19417
    Illegal NheI site found at 10607
    Illegal NheI site found at 15318
    Illegal PstI site found at 6102
    Illegal PstI site found at 7554
    Illegal PstI site found at 10344
    Illegal PstI site found at 10356
    Illegal NotI site found at 7962
    Illegal NotI site found at 14619
    Illegal NotI site found at 15909
    Illegal NotI site found at 17442
    Illegal NotI site found at 19405
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6075
    Illegal EcoRI site found at 7974
    Illegal EcoRI site found at 19417
    Illegal BamHI site found at 4344
    Illegal BamHI site found at 6362
    Illegal BamHI site found at 10386
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6075
    Illegal EcoRI site found at 7974
    Illegal EcoRI site found at 19417
    Illegal XbaI site found at 10380
    Illegal PstI site found at 6102
    Illegal PstI site found at 7554
    Illegal PstI site found at 10344
    Illegal PstI site found at 10356
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6075
    Illegal EcoRI site found at 7974
    Illegal EcoRI site found at 19417
    Illegal XbaI site found at 10380
    Illegal PstI site found at 6102
    Illegal PstI site found at 7554
    Illegal PstI site found at 10344
    Illegal PstI site found at 10356
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 5255
    Illegal NgoMIV site found at 11335
    Illegal NgoMIV site found at 13073
    Illegal NgoMIV site found at 14536
    Illegal NgoMIV site found at 15517
    Illegal NgoMIV site found at 15662
    Illegal AgeI site found at 4752
    Illegal AgeI site found at 6342
    Illegal AgeI site found at 14824
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1335
    Illegal SapI.rc site found at 7986
    Illegal SapI.rc site found at 11184
    Illegal SapI.rc site found at 11394
    Illegal SapI.rc site found at 14229
    Illegal SapI.rc site found at 19429
    Illegal SapI site found at 14692


[edit]
Categories
Parameters
None