Composite

Part:BBa_K3807033

Designed by: Viktoriia Belousova   Group: iGEM21_KU_Leuven   (2021-10-01)


pGGK-p35S-NLS-N7-enCas9-PolI3M-TBD-P2A-mCherry-sacB

pGG plasmid containing Evolvr enCas9-PolI3M-TBD expression cassette (BBa_K380707030) followed by a SacB promoter and SacB gene flanked by AarI restriction sites so that it can be easily substituted by a custom gRNA expression cassette

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9062
    Illegal EcoRI site found at 17309
    Illegal XbaI site found at 25
    Illegal PstI site found at 1
    Illegal PstI site found at 15437
    Illegal PstI site found at 16889
    Illegal PstI site found at 19679
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9062
    Illegal EcoRI site found at 17309
    Illegal NheI site found at 252
    Illegal NheI site found at 4963
    Illegal PstI site found at 1
    Illegal PstI site found at 15437
    Illegal PstI site found at 16889
    Illegal PstI site found at 19679
    Illegal NotI site found at 4264
    Illegal NotI site found at 5554
    Illegal NotI site found at 7087
    Illegal NotI site found at 9050
    Illegal NotI site found at 17297
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9062
    Illegal EcoRI site found at 17309
    Illegal BamHI site found at 31
    Illegal BamHI site found at 13472
    Illegal BamHI site found at 15697
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9062
    Illegal EcoRI site found at 17309
    Illegal XbaI site found at 25
    Illegal PstI site found at 1
    Illegal PstI site found at 15437
    Illegal PstI site found at 16889
    Illegal PstI site found at 19679
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9062
    Illegal EcoRI site found at 17309
    Illegal XbaI site found at 25
    Illegal PstI site found at 1
    Illegal PstI site found at 15437
    Illegal PstI site found at 16889
    Illegal PstI site found at 19679
    Illegal NgoMIV site found at 980
    Illegal NgoMIV site found at 2718
    Illegal NgoMIV site found at 4181
    Illegal NgoMIV site found at 5162
    Illegal NgoMIV site found at 5307
    Illegal NgoMIV site found at 5311
    Illegal NgoMIV site found at 5518
    Illegal AgeI site found at 4469
    Illegal AgeI site found at 13880
    Illegal AgeI site found at 15677
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 829
    Illegal SapI.rc site found at 1039
    Illegal SapI.rc site found at 3874
    Illegal SapI.rc site found at 9074
    Illegal SapI.rc site found at 10463
    Illegal SapI.rc site found at 17321
    Illegal SapI site found at 4337


[edit]
Categories
Parameters
None