Composite

Part:BBa_K3807030

Designed by: Sarah Vorsselmans   Group: iGEM21_KU_Leuven   (2021-10-01)


plant enCas9-PolI3MTBD expression cassette

EvolvR enCas9-PolI3M-TBD fused with N-7 NLS at the N-terminus, followed by P2A self-cleaving peptide and fluorescent protein mCherry wiht NLS as transformation marker. Expression is regulated by CamV35 promoter and terminator

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 884
    Illegal EcoRI site found at 9131
    Illegal PstI site found at 7259
    Illegal PstI site found at 8711
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 884
    Illegal EcoRI site found at 9131
    Illegal PstI site found at 7259
    Illegal PstI site found at 8711
    Illegal NotI site found at 872
    Illegal NotI site found at 9119
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 884
    Illegal EcoRI site found at 9131
    Illegal BamHI site found at 5294
    Illegal BamHI site found at 7519
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 884
    Illegal EcoRI site found at 9131
    Illegal PstI site found at 7259
    Illegal PstI site found at 8711
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 884
    Illegal EcoRI site found at 9131
    Illegal PstI site found at 7259
    Illegal PstI site found at 8711
    Illegal NgoMIV site found at 2204
    Illegal NgoMIV site found at 6205
    Illegal AgeI site found at 5702
    Illegal AgeI site found at 7499
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 896
    Illegal SapI.rc site found at 2285
    Illegal SapI.rc site found at 9143


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Categories
Parameters
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