Composite

Part:BBa_K3807029

Designed by: Viktoriia Belousova   Group: iGEM21_KU_Leuven   (2021-10-01)


plant enCas9-PolI5M expression cassette

EvolvR enCas9-PolI5M fused with N-7 NLS at the N-terminus, followed by P2A self-cleaving peptide and fluorescent protein mCherry wiht NLS as transformation marker. Expression is regulated by CamV35 promoter and terminator

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 884
    Illegal EcoRI site found at 8924
    Illegal PstI site found at 7052
    Illegal PstI site found at 8504
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 884
    Illegal EcoRI site found at 8924
    Illegal PstI site found at 7052
    Illegal PstI site found at 8504
    Illegal NotI site found at 872
    Illegal NotI site found at 8912
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 884
    Illegal EcoRI site found at 8924
    Illegal BamHI site found at 5294
    Illegal BamHI site found at 7312
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 884
    Illegal EcoRI site found at 8924
    Illegal PstI site found at 7052
    Illegal PstI site found at 8504
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 884
    Illegal EcoRI site found at 8924
    Illegal PstI site found at 7052
    Illegal PstI site found at 8504
    Illegal NgoMIV site found at 2204
    Illegal NgoMIV site found at 6205
    Illegal AgeI site found at 5702
    Illegal AgeI site found at 7292
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 896
    Illegal SapI.rc site found at 2285
    Illegal SapI.rc site found at 8936


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Categories
Parameters
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