Coding

Part:BBa_K3807027

Designed by: Viktoriia Belousova   Group: iGEM21_KU_Leuven   (2021-10-01)


GS gRNA expression cassette 2

protospacer targeting BY-2 glutamine synthetase (GS) gene at the BASTA binding site in the 11th exon merged with enCas9 gRNA body and regulated by AtU6-26 promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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