Plasmid

Part:BBa_K3807025

Designed by: Viktoriia Belousova   Group: iGEM21_KU_Leuven   (2021-10-01)


pGGK

Standard plant Golden Gate assembly backbone carrying T-dna borders, kanamycin resistance gene expressed in plats and spectinomycin resistance expressed in bacteria

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7820
    Illegal XbaI site found at 8182
    Illegal PstI site found at 8158
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7820
    Illegal NheI site found at 3503
    Illegal NheI site found at 8409
    Illegal PstI site found at 8158
    Illegal NotI site found at 2804
    Illegal NotI site found at 4094
    Illegal NotI site found at 5627
    Illegal NotI site found at 8139
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7820
    Illegal BamHI site found at 7367
    Illegal BamHI site found at 8070
    Illegal BamHI site found at 8188
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7820
    Illegal XbaI site found at 8182
    Illegal PstI site found at 8158
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7820
    Illegal XbaI site found at 8182
    Illegal PstI site found at 8158
    Illegal NgoMIV site found at 1258
    Illegal NgoMIV site found at 2721
    Illegal NgoMIV site found at 3702
    Illegal NgoMIV site found at 3847
    Illegal NgoMIV site found at 3851
    Illegal NgoMIV site found at 4058
    Illegal NgoMIV site found at 4280
    Illegal AgeI site found at 3009
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8147
    Illegal BsaI.rc site found at 6720
    Illegal SapI.rc site found at 2414
    Illegal SapI.rc site found at 8986
    Illegal SapI.rc site found at 9196
    Illegal SapI site found at 2877


[edit]
Categories
Parameters
None