Coding

Part:BBa_K3807024

Designed by: Sarah Vorsselmans   Group: iGEM21_KU_Leuven   (2021-10-01)


SacB

SacB encodes the enzyme levansucrase from Bacillus subtilis that converts sucrose into a toxic metabolite in gram-negative bacteria. Can be used as negative selection at cloning sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None