Composite

Part:BBa_K3807018

Designed by: Sarah Vorsselmans   Group: iGEM21_KU_Leuven   (2021-10-01)


Riboswitch + selection device

Riboswitch device to be inserted in a low-copy number plasmid. It includes an indicable promoter (BBa_I0500), theophylline riboswitch (BBa_K598005) and TetA (BBa_J31007)-sfGFP fusion with flexible linker. This device allows for the positive and negative selection of protein expression (through the TetA gene).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2516
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2516
    Illegal NheI site found at 1205
    Illegal NheI site found at 1441
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2516
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1587
    Illegal BamHI site found at 2486
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2516
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2516
    Illegal NgoMIV site found at 1613
    Illegal NgoMIV site found at 1981
    Illegal NgoMIV site found at 2141
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2672
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
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