Coding

Part:BBa_K3807003

Designed by: Viktoriia Belousova   Group: iGEM21_KU_Leuven   (2021-10-01)


codon harmonized enCas9-PolI5M

Variant of the EvolvR complex consisting of enhanced Cas9 enzyme fused to DNA polymerase I variant 5M, codon harmonized for expression in Nicotiniana tabacum Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1111
    Illegal XbaI site found at 300
    Illegal XbaI site found at 1818
    Illegal XbaI site found at 3795
    Illegal PstI site found at 790
    Illegal PstI site found at 2212
    Illegal PstI site found at 5686
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1111
    Illegal PstI site found at 790
    Illegal PstI site found at 2212
    Illegal PstI site found at 5686
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1111
    Illegal BglII site found at 3815
    Illegal BamHI site found at 2207
    Illegal XhoI site found at 1138
    Illegal XhoI site found at 1377
    Illegal XhoI site found at 2328
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1111
    Illegal XbaI site found at 300
    Illegal XbaI site found at 1818
    Illegal XbaI site found at 3795
    Illegal PstI site found at 790
    Illegal PstI site found at 2212
    Illegal PstI site found at 5686
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1111
    Illegal XbaI site found at 300
    Illegal XbaI site found at 1818
    Illegal XbaI site found at 3795
    Illegal PstI site found at 790
    Illegal PstI site found at 2212
    Illegal PstI site found at 5686
    Illegal NgoMIV site found at 6629
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 22
    Illegal BsaI site found at 4060
    Illegal BsaI.rc site found at 848
    Illegal BsaI.rc site found at 3202
    Illegal BsaI.rc site found at 4557
    Illegal SapI.rc site found at 1159
    Illegal SapI site found at 1653
    Illegal SapI site found at 3768


[edit]
Categories
Parameters
None