Coding

Part:BBa_K3807001

Designed by: Viktoriia Belousova   Group: iGEM21_KU_Leuven   (2021-10-01)


enCas9-PolI3M

Variant of the EvolvR complex consisting of enhanced Cas9 enzyme fused to DNA polymerase I variant 3M.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5899
    Illegal PstI site found at 5926
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5899
    Illegal PstI site found at 5926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5899
    Illegal BamHI site found at 4168
    Illegal BamHI site found at 6186
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5899
    Illegal PstI site found at 5926
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5899
    Illegal PstI site found at 5926
    Illegal NgoMIV site found at 1078
    Illegal NgoMIV site found at 5079
    Illegal AgeI site found at 4576
    Illegal AgeI site found at 6166
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1159


[edit]
Categories
Parameters
None