Signalling

Part:BBa_K3804009

Designed by: iGEM21_UTokyo   Group: iGEM21_UTokyo   (2021-09-25)


Reporter for quorum sensing systems in yeast with qscR

We created this part with reference to BBa_K1024002 in order to signal when Pseudomonas aeruginosa exists. The part is composed of pTEF, Flag, VP16, NLS, QscR, ADH1 terminator, pQsc, Cyc100mini, mCherry and CYC1terminator. We improved BBa_K1024002 by adding ADH1 terminator and CYC1terminator　and changed LuxR to QscR, pLux to pQsc. QscR is homolog of LusR. The QscR gene is constitutively expressed. On the other hands, activation of pQsc occurs only when sufficient amount of AHL exist in the environment. When pQsc was activated, mCherry is expressed and lets us know the existence of AHL.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1673
    Illegal XhoI site found at 1595
    Illegal XhoI site found at 1646
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1811



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Parameters
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