Coding
SOD1

Part:BBa_K380007

Designed by: Andreas Constantinou, Emmelie Lidh, Nina Schiller, Johan Nordholm   Group: iGEM10_Stockholm   (2010-10-25)

Superoxide dismutase 1 protein

Human soluble superoxide dismutase 1 (SOD1) is a soluble cytoplasmic protein functional as a homodimer that binds copper and zink ions. SOD1 catalyzes the reaction O-2 + O-2 + 2H+ → H2O2 + O2, protecting the cell from oxidative damage. SOD1 was first cloned and expressed in E. coli by [http://www.ncbi.nlm.nih.gov/pubmed/3889846 Hallewell et al., (1985)]. Co-expression of SOD1 with the yeast copper chaperon (yCCS) ([http://www.ncbi.nlm.nih.gov/pubmed/15358352 Ahl et al. 2003]) yields proteins with higher copper contents, leading to increased activity and more stable proteins.

For the helper yeast copper chaperone (yCCS), see Part:BBa_K380008.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 323


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Categories
Parameters
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