Part:BBa_K380006

Human basic fibroblast growth factor, bFGF

Recombinant human fibroblast growth factor, bFGF - also abbreviated FGF-2 and FGF-ß, is a member of fibroblast growth factor family[1] and can be produced in E. coli as a single, non-glycosylated protein[2]. In humans, different reports shows a predominant localization both in the nucleus and the cytosol, with unknown reason for inconsistency[3].

In skin, melanocyte and keratinocyte cells form the so-called epidermo-melanic unit. This is partly mediated by cytokines derived from the keratinocytes, which are important for the well-being of melanocytes. One of the cytokines secreted by keratinocytes is bFGF, acting as a mitogen for melanocytes and also promotes melanin synthesis[4].

[1] Fibroblast growth factors, 2001

[2] Expression and purification of a biologically active basic fibroblast growth factor fusion protein, 2003

[3] Nuclear and Nucleolar Localization of 18-kDa Fibroblast Growth Factor-2 Is Controlled by C-terminal Signals, 2003

[4] New Insights into the Pathogenesis of Vitiligo: Imbalance of Epidermal Cytokines at Sites of Lesions, 2002

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 403
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 159