Part:BBa_K3800016
Regulatory binding site for LwCas13a protein. Used with RNA sequence to target a specific gene/miRNA
Cas systems are used by bacteria and archaea as an adaptive immunity against viruses and plasmids, and crRNAs are important to guide the silencing of invading nucleic acids. The pin-loop structure binds to the Cas protein, while the remainder of the sequence is complementary to a specific RNA sequence and allows the Cas protein to target that sequence. Similarly to the Cas9 protein, Cas13a uses a ~64 nucleotide guide RNA to encode target specificity. The Cas13a protein recognizes and forms a complex with the guide RNA via the pin-loop structure in the crRNA, while target specificity is encoded by the remainder of the crRNA as a 28-30 nt spacer complementary to the target. Once the Cas13-crRNA complex has paired with the target RNA, the Cas complex is able to cleave the target molecule and promote degradation of the target molecule. The cleavage activity of Cas13a is activated by complementary base pairing with the target RNA. In the absence of base-pairing, the Cas13a protein exhibits a negligible amount of nuclease activity.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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