Composite

Part:BBa_K3799034

Designed by: Shubhamay Das   Group: iGEM21_IISER_Kolkata   (2021-10-19)


pBAD strong Promoter with I746100 (AIP sensor infrastructure - agrC + agrA with RBSes) + B0015 (Term

pBAD is an E.coli promoter that is induced by L-arabinose. Under this promoter we have put the AIP sensor infrastructure (I746100) from the agr system with a standard registry terminator (B0015) downstream.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None