Composite

Part:BBa_K3796216

Designed by: Feiyue Yang   Group: iGEM21_CAU_China   (2021-10-01)


RNAi route for odhA repression

This composite part was designed to work as our RNAi test circuit. Our insert is only under the control of Ptac and lac operator. Basically, in the presence of Isopropyl β-D-1-thiogalactopyranoside (IPTG), the transcript of our insert will come out and forbid odhA mRNAs being translated as antisense RNAs. As a result, odhA is knocked down and more glutamate is manufactured.
In theory, the COMPLETE insert is not necessary here.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3735
    Illegal PstI site found at 1635
    Illegal PstI site found at 2940
    Illegal PstI site found at 3213
    Illegal PstI site found at 3252
    Illegal PstI site found at 3312
    Illegal PstI site found at 4508
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3735
    Illegal PstI site found at 1635
    Illegal PstI site found at 2940
    Illegal PstI site found at 3213
    Illegal PstI site found at 3252
    Illegal PstI site found at 3312
    Illegal PstI site found at 4508
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3735
    Illegal BglII site found at 3683
    Illegal BglII site found at 4244
    Illegal XhoI site found at 3300
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3735
    Illegal PstI site found at 1635
    Illegal PstI site found at 2940
    Illegal PstI site found at 3213
    Illegal PstI site found at 3252
    Illegal PstI site found at 3312
    Illegal PstI site found at 4508
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3735
    Illegal PstI site found at 1635
    Illegal PstI site found at 2940
    Illegal PstI site found at 3213
    Illegal PstI site found at 3252
    Illegal PstI site found at 3312
    Illegal PstI site found at 4508
    Illegal NgoMIV site found at 1263
    Illegal NgoMIV site found at 4474
    Illegal AgeI site found at 3621
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2217
    Illegal BsaI site found at 2366
    Illegal BsaI site found at 2409
    Illegal BsaI site found at 3417
    Illegal BsaI site found at 3483
    Illegal BsaI.rc site found at 1387
    Illegal BsaI.rc site found at 2911
    Illegal SapI site found at 2198


[edit]
Categories
Parameters
None