Composite

Part:BBa_K3793012

Designed by: Rupesh Balaji Jayachandran, Enrique de Dios Mateos   Group: iGEM21_Aalto-Helsinki   (2021-10-21)


sfGFP reporter

This composite part is made from combining the coding sequence of a superfolder GFP, T7 transcription terminator and a CYC1 transcription terminator.

This composite plug is intended to be used as a reporter for promoter activity in a bacterial T7 expression system or Saccharomyces cerevisiae. There are BsaI restriction sites upstream of the sfGFP sequence to insert the promoter that is to be analyzed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 787
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 7
    Illegal XhoI site found at 480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 35
    Illegal BsaI.rc site found at 19


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Categories
Parameters
None