Coding

Part:BBa_K3793011

Designed by: Rupesh Balaji Jayachandran, Enrique de Dios Mateos   Group: iGEM21_Aalto-Helsinki   (2021-10-21)


GST tag with enterokinase cleavage site

The sequence encodes for a Glutathione S-Transferase (GST) tag with an enterokinase tag (DDDDK). GST tag can be added to a protein's coding sequence for purification. GST tags can also improve the solubility of the protein. After purification the GST tag can be removed through enterokinase enzymes which recognize the 'DDDDK' peptide and proceed to cleave.

The part contains the enterokinase site at the N-Terminal of GST intended for C-Terminal tagging of target protein. This part needs to be added before the stop codon of the target protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 100


[edit]
Categories
Parameters
None