Coding

Part:BBa_K3793006

Designed by: Rupesh Balaji Jayachandran, Enrique de Dios Mateos   Group: iGEM21_Aalto-Helsinki   (2021-10-21)


CDS - Human AhR Interacting Protein

This sequence encodes for the full length human Aryl hydrocarbon receptor Interacting Protein (AIP). AIP is an interacting partner of the Aryl hydrocarbon receptor (AhR). AIP shields the DNA binding domain of AhR. Upon ligand binding, AIP dissociates allowing for the nuclear translocation of AhR.

The part contains the coding sequence for the full length AIP containing the FKBP52-like domain and the three TPR domains. The CDS has been codon optimized to remove any type IIS restriction enzyme sites. An N-Terminal 6x HIS tag is added to the CDS.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 439
    Illegal PstI site found at 652
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 439
    Illegal PstI site found at 652
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 53
    Illegal BamHI site found at 686
    Illegal BamHI site found at 953
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 439
    Illegal PstI site found at 652
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 439
    Illegal PstI site found at 652
    Illegal AgeI site found at 578
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 779


[edit]
Categories
Parameters
None