Regulatory

Part:BBa_K3793000

Designed by: Rupesh Balaji Jayachandran, Enrique de Dios Mateos   Group: iGEM21_Aalto-Helsinki   (2021-10-20)


T7 promoter

The part contains the sequence for the T7 promoter optimal for protein expression in bacteria using the T7 RNA polymerase system. This promoter is suitable for transcription in common laboratory Escherichia coli strains like BL21 and C2566.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 33
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 36


[edit]
Categories
Parameters
None