Coding

Part:BBa_K3788007

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)


6His_ConA

BBa_K3788007 sequence is coding for ConcanavalinA (ConA), a glycoprotein able to interact with enveloped viruses. 6His_conA have a 6his tag in Nter to allow to purified proteins; and allow the detection and specific identification by WesterBlot.

Sequence was optimised for E. coli K12.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 165
    Illegal XhoI site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 427
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None