Coding

Part:BBa_K3786003

Designed by: Nathalia Raquel de Souza Fernandes   Group: iGEM21_Paris_Bettencourt   (2021-10-21)


Min operon (min C, D, E)

The min operon in E.coli is composed of three genes: minC, minD and minE respectively encoding for the proteins MinC, MinD and MinE. More precisely, MinC is one of the main negative regulators of FtsZ by preserving FtsZ polymerization. And overall, this Min system helps to center the position of the Z-ring by restricting FtsZ assembly to the middle of the cell thanks to a high concentration of FtsZ polymerization inhibitor at the pole of the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 199
    Illegal PstI site found at 1146
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 199
    Illegal PstI site found at 1146
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 199
    Illegal PstI site found at 1146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 199
    Illegal PstI site found at 1146
    Illegal NgoMIV site found at 821
    Illegal AgeI site found at 404
    Illegal AgeI site found at 700
    Illegal AgeI site found at 1587
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 9
    Illegal SapI site found at 567


[edit]
Categories
Parameters
None