Composite

Part:BBa_K3782026

Designed by: Jana Näf   Group: iGEM21_UNILausanne   (2021-10-20)


Cas9 with sgRNA 2 and donor sequence

This composite part contains the Cas9 protein, sgRNA sequence, and donor sequence. It can be used as a part of the CRISPR/Cas9 technology, which is derived from the adaptive immune system of many prokaryotes. Once produced, the Cas9 protein will be associated with a single-guide RNA (sgRNA), which is complementary to the DNA sequence we aim to target and cut. If the sgRNA finds its complementary sequence, Cas9 will induce a double-strand break in proximity of it, opening the DNA double helix.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1072
    Illegal BglII site found at 1762
    Illegal BglII site found at 4597
    Illegal BamHI site found at 3404
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4574
    Illegal NgoMIV site found at 5714
    Illegal AgeI site found at 4861
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1679
    Illegal SapI.rc site found at 4407


[edit]
Categories
Parameters
None