Part:BBa_K3782002

6XHis-Tag

6xHis-Tag for Purification and Identification of Protein of Interest

The His-Tag is a commonly used sequence both as a reporter and as a purification tag. Its sequence, consisting of 6 histidine residues, has a very high affinity to nickel(II) ions and is therefore used in affinity purification columns with immobilized nickel ions. The binding of the His-Tag to the nickel ions is not efficient at acidic pH values, and any purification process should therefore be done above pH=7. Imidazole is used to elute the protein from the column by competing with the His tag on binding to the immobilized Nickel ions.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]