Composite
WT Dsup

Part:BBa_K3780011

Designed by: Sumohit Sharma   Group: iGEM21_Stuttgart   (2021-09-27)


Wild type Dsup Sequence

The DNA fragment BBa_3780011 contains the information for the DSUP protein. DSUP is a protein that is unique to tardigrades. It protects DNA from UV and ROS damage. Snapgene was used to build in silico the BioBricks and plasmid DSUP construct. The fragments were cloned into the pBAD18 by Gibson Assembly. Two different E. Coli strains were transformed with the fragment (DH5alpha, CW25113) to test the UV protection potential of DSUP in E. Coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1562
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 203
    Illegal NheI site found at 293
    Illegal SpeI site found at 1563
    Illegal PstI site found at 1577
    Illegal NotI site found at 7
    Illegal NotI site found at 1570
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 142
    Illegal BamHI site found at 1175
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1563
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1563
    Illegal PstI site found at 1577
    Illegal NgoMIV site found at 1511
  • 1000
    COMPATIBLE WITH RFC[1000]


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