Composite

Part:BBa_K3778034

Designed by: Yun Tsai   Group: iGEM21_CCU_Taiwan   (2021-09-06)


T7P-RBS-6xHis-Enterokinase cleavage site-CBD-linker-Thrombin releasable TAT 48-57 -T7T

The synthetic protein from this composite part is applied to examine whether the removal of the 47th amino acid Tyrosine (Y) in TAT (BBa_K1202006) increases the probability of Thrombin mediated release.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 761
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 761
    Illegal NheI site found at 824
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 761
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 761
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 761
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None