Coding

Part:BBa_K377705

Designed by: Marcus Lehr   Group: iGEM10_Michigan   (2010-10-26)

Flu (Ag43)

The flu operon in E. coli codes for the Antigen 43 complex (Ag43). This is a self-inserting, self-dimerizing surface membrane protein, which is involved in autoaggregation, adhesiveness, and biofilm formation in E. coli. It has also been shown to be fully functional in P. fluorescens [1].

The part was amplified from E. coli using previously described primers [1], which were modified to contain BioBrick prefix and suffixes. It was then cloned into the pSB1AT3 backbone, with the intention of also cloning in the J23100 constitutive promoter and expressing the construct in P. fluorescens and P. putida to assay for increased biofilm formation.


1. [http://jb.asm.org/cgi/content/short/182/17/4789 Antigen 43 from Escherichia coli Induces Inter- and Intraspecies Cell Aggregation and Changes in Colony Morphology of Pseudomonas fluorescens]

Sequence sources can be found on the part design page.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 883
    Illegal PstI site found at 1441
    Illegal PstI site found at 2041
    Illegal PstI site found at 2665
    Illegal PstI site found at 2936
    Illegal PstI site found at 2989
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 883
    Illegal PstI site found at 1441
    Illegal PstI site found at 2041
    Illegal PstI site found at 2665
    Illegal PstI site found at 2936
    Illegal PstI site found at 2989
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 883
    Illegal PstI site found at 1441
    Illegal PstI site found at 2041
    Illegal PstI site found at 2665
    Illegal PstI site found at 2936
    Illegal PstI site found at 2989
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 883
    Illegal PstI site found at 1441
    Illegal PstI site found at 2041
    Illegal PstI site found at 2665
    Illegal PstI site found at 2936
    Illegal PstI site found at 2989
    Illegal NgoMIV site found at 709
    Illegal NgoMIV site found at 1933
    Illegal NgoMIV site found at 2443
    Illegal NgoMIV site found at 2464
    Illegal AgeI site found at 1459
    Illegal AgeI site found at 2203
    Illegal AgeI site found at 2617
    Illegal AgeI site found at 2859
  • 1000
    COMPATIBLE WITH RFC[1000]


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