Composite
CF clpB se

Part:BBa_K3773528

Designed by: Avery Bradley, Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-18)


Circuit to report PclpB expression in vitro

This circuit is a version of BBa_K3773518 optimized for in vitro use. The circuit places sfGFP before the clpB promoter, allowing it to report expression levels of the heat shock response gene clpB, which is normally controlled by PclpB in E. coli.

We use this circuit to compare PclpB expression in cells that have either been transformed or not been transformed with a certain circuit.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 275
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 461


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Categories
Parameters
None