Composite
CF lon sen

Part:BBa_K3773527

Designed by: Avery Bradley, Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-18)


Circuit to report Plon expression in vitro

This circuit is a version of BBa_K3773523 optimized for in vitro use. The circuit places sfGFP before the lon promoter, allowing it to report expression levels of the protease gene lon, which is normally controlled by Plon in E. coli.

We use this circuit to compare Plon expression in cells that have either been transformed or not been transformed with a certain circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 324


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Categories
Parameters
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