Composite
CF TL sens

Part:BBa_K3773526

Designed by: Avery Bradley   Group: iGEM21_William_and_Mary   (2021-10-18)


Circuit to report translation in vitro

This sensor circuit uses sfGFP to report general levels of translation in E. coli in vitro. We use this translational sensor to detect changes in host translation when an additional circuit is added. This sensor encodes sfGFP under the control of the constitutive promoter p70a and associated RBS. As the fluorescence output of this circuit is a direct cause of translation, it can be used as a measure of circuit interactions with host translation. Decreases in sfGFP fluorescence with and without the desired tested circuit will reflect the impact of the additional circuit on hosts functions, reflecting host-circuit orthogonality levels.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 102
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 155


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Categories
Parameters
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