Composite
CF TX sens

Part:BBa_K3773525

Designed by: Avery Bradley   Group: iGEM21_William_and_Mary   (2021-10-15)


Circuit to report transcription in vitro

This circuit places a green fluorescent aptamer after a constitutive promoter, allowing us to quantify the level of transcription occurring in a host cell at a given time. The circuit is designed for optimal use in vitro.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 191
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 102
    Illegal SpeI site found at 191
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 191
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 191
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None