Composite
hslVU sens

Part:BBa_K3773524

Designed by: Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-21)


Circuit to report PhslVU expression in vivo

This circuit places sfGFP before the hslVU promoter, allowing it to report expression levels of the protease complex hslVU, which is normally controlled by PhslVU in E. coli.

We use this circuit to compare PhslVU expression in cells that have either been transformed or not been transformed with a certain circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 296
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None