Composite
lon sensor

Part:BBa_K3773523

Designed by: Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-15)


Circuit to report Plon expression in vivo

This circuit places sfGFP before the lon promoter, allowing it to report expression levels of the protease gene lon, which is normally controlled by Plon in E. coli.

We use this circuit to compare Plon expression in cells that have either been transformed or not been transformed with a certain circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 459
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None