Composite
dnaKJ sens
Part:BBa_K3773521
Designed by: Pinar Banu Caglayan Group: iGEM21_William_and_Mary (2021-10-15)
Circuit to report PdnaKJ expression in vivo
This circuit places sfGFP before the dnaKJ operon promoter, allowing it to report expression levels of the heat shock response genes dnaK and dnaJ, which are normally controlled by the promoter in E. coli.
We use this circuit to compare PdnaK/dnaJ expression in cells that have either been transformed or not been transformed with a certain circuit.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 367
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |