Composite
dnaKJ sens

Part:BBa_K3773521

Designed by: Pinar Banu Caglayan   Group: iGEM21_William_and_Mary   (2021-10-15)


Circuit to report PdnaKJ expression in vivo

This circuit places sfGFP before the dnaKJ operon promoter, allowing it to report expression levels of the heat shock response genes dnaK and dnaJ, which are normally controlled by the promoter in E. coli.

We use this circuit to compare PdnaK/dnaJ expression in cells that have either been transformed or not been transformed with a certain circuit.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 367
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None