Composite
clpB senso

Part:BBa_K3773517

Designed by: Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-15)


Circuit to report PclpB expression in vivo

This sensor places sfGFP after a promoter which normally controls the expression of the heat shock protein ClpB in E. coli. Therefore, cells transformed with this circuit report expression levels of the clpB promoter via level of green fluorescence.

We use this circuit to compare PclpB expression in cells that have either been transformed or not been transformed with a certain circuit.

This circuit has an alternate form, BBa_B7337518.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 275
    Illegal AgeI site found at 587
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None