Composite
clpB senso
Part:BBa_K3773517
Designed by: Justin Berg Group: iGEM21_William_and_Mary (2021-10-15)
Circuit to report PclpB expression in vivo
This sensor places sfGFP after a promoter which normally controls the expression of the heat shock protein ClpB in E. coli. Therefore, cells transformed with this circuit report expression levels of the clpB promoter via level of green fluorescence.
We use this circuit to compare PclpB expression in cells that have either been transformed or not been transformed with a certain circuit.
This circuit has an alternate form, BBa_B7337518.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 275
Illegal AgeI site found at 587 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |