Composite
TL sensor

Part:BBa_K3773516

Designed by: Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-15)


Circuit to report translation in vivo

This sensor circuit uses sfGFP to report general levels of translation in E. coli. We use this translational sensor to detect changes in host translation when an additional circuit is added. This sensor uses the design employed by Ceroni et al,, 2015. As the fluorescence output of this circuit is a direct cause of translation, it can be used as a measure of circuit interactions with host translation. Decreases in sfGFP fluorescence with and without the desired tested circuit will reflect the impact of the additional circuit on hosts functions, reflecting host-circuit orthogonality levels.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 868
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
    Illegal SpeI site found at 868
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 868
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 868
    Illegal AgeI site found at 255
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None