Part:BBa_K3773511

Circuit to report transcription in vivo

This part places a green fluorescent aptamer, F30-Broccoli, after a constitutive promoter. This circuit is designed to report general transcriptional levels in vivo in E. coli. As the fluorescence output of this circuit is a direct cause of transcription, it can be used as a measure of a second, test circuit's impact on the host's capacity to transcribe DNA.

Once the correct aptamer dye has been applied to cells transformed with this circuit, a fluorescence output will be produced from the aptamer. As the circuit does not include an RBS, this circuit is able to detect decreases in burden on the transcriptional level. Therefore, based on the amount of aptamer transcribed, the fluorescence output will fluctuate, providing a measure of the test circuit’s impact on the host.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 47
  Illegal NheI site found at 70
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]