DNA
Spinach2

Part:BBa_K3773013

Designed by: Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-15)


Spinach2 aptamer

This is the Spinach2 aptamer, which fluoresces green upon binding to the appropriate fluorophore. We use this aptamer as a fluorescence reporter in BBa_K3773525, our circuit to sense transcriptional levels in vitro.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 84
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 84
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 84
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 84
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None