Regulatory
PdnaKJ

Part:BBa_K3773009

Designed by: Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-15)


dnaKJ operon promoter/RBS

This is the dnaKJ operon promoter/RBS-containing region native to E. coli. We use this in our composite part BBa_K3773521 in order to report expression levels of the heat shock response genes dnaK and dnaJ, which are normally controlled by the promoter/RBS in E. coli.

This sequence includes the RBS native to E. coli after the promoter and any spacers required; as a result, it can be placed directly before the CDS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None