Regulatory
PdnaKJ
Part:BBa_K3773009
Designed by: Justin Berg Group: iGEM21_William_and_Mary (2021-10-15)
dnaKJ operon promoter/RBS
This is the dnaKJ operon promoter/RBS-containing region native to E. coli. We use this in our composite part BBa_K3773521 in order to report expression levels of the heat shock response genes dnaK and dnaJ, which are normally controlled by the promoter/RBS in E. coli.
This sequence includes the RBS native to E. coli after the promoter and any spacers required; as a result, it can be placed directly before the CDS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |