Coding
sfGFP

Part:BBa_K3773003

Designed by: Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-14)


Codon-optimized sfGFP

This is a codon-optimized version of the superfolder green fluorescence protein. The sequence was provided in the supplementary information of Ceroni et al.'s 2015 paper, "Quantifying cellular capacity identifies gene expression designs with reduced burden." We use this protein to report expression levels of various promoters, allowing us to compare expression between E. coli cells transformed with a certain genetic circuit and cells that are not transformed with the circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None