Regulatory

Part:BBa_K3772019

Designed by: Han Zonglin   Group: iGEM21_SCUT-China   (2021-10-01)


SOL4 promoter from S. cerevisiae

The fermentation results and corresponding yield of valencene when SOL4p(BBa_K3772019) is transferred into the S. cerevisiae are shown below.
T--SCUT China--wiki2.jpg
T--SCUT China--wiki3.jpg

Electrophoretic images are also shown below, and the seventh lane represents SOL4p
T--SCUT China--wiki12.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 430
    Illegal XbaI site found at 522
    Illegal SpeI site found at 534
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 430
    Illegal SpeI site found at 534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 430
    Illegal XhoI site found at 316
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 430
    Illegal XbaI site found at 522
    Illegal SpeI site found at 534
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 430
    Illegal XbaI site found at 522
    Illegal SpeI site found at 534
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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