Plasmid

Part:BBa_K3769101

Designed by: Jiale Hong   Group: iGEM21_FZU-China   (2021-10-02)


loxP-T500(2)-T500(2)-loxP-T7RBS-gadB-T7RBS-φX174E

Cre-Vvd will identify and cut off the gene between two loxP sites to express gadB and φX174E.gadB make glutamate turn to GABA.In order to make E. coli release gadB more effectively, we use φX174E to achieve cytolysis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 889
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None